[Histonet] Re: Histonet Digest, Vol 20, Issue 14

From:Amos Brooks

Rich & Andi,
    Rich, I'd like to second Andi's recommendation for Sigma as a source 
for Fast Myosin although we don't bother with HIER. Give it a whirl both 
ways, you never know her's might be better :-)
    Andi, while you asked Rich I figured I'd chime in for Dako as a 
source for Hepatitis (core & surface). Unfortunately I'm out of the lab 
right now so I don't have the cat #s. If  you need them just drop me a 
line and I'll rummage them up.
Good luck guys,


Message: 22
Date: Tue, 12 Jul 2005 16:37:08 +0200
From: "Andi Kappeler" 
Subject: Re: [Histonet] Fast myosin IHC
To: "Richard Cartun" ,	"Histonet"
Message-ID: <008801c586ef$40bf45f0$27955c82@patho.unibe.ch>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";

Hi Richard

We successfully use:
- mo-a-Myosin II (fast), clone MY-32, Sigma M 4276, working conc. approx 10 
ug Ig/ml (corresponds to 1:400 with the lot we have), HIER in citrate pH 6.0 
(pressure cooker)
- mo-a-Myosin I (slow), clone NOQ7.5.4D, Sigma M 8421, working conc. approx 
7.5 ug Ig/ml (corresponds to 1:1000 with the lot we have), HIER in citrate 
pH 6.0 (pressure cooker)
Both mAbs work very well (see 
http://www.pathology.unibe.ch/Diagn/speztech/spez_ihc.htm for a pic (german 
website, sorry).

Can you give me a hint in return, where you get your antibodies against 
Hepatitis B surface Ag (HBs) and core Ag (HBc) from? Our source is no longer 
able to supply them, due to some bureaucratic / eurocratic rules, now I'm 
looking for a reliable replacement.
Many thanks in advance!

Best regards
Andi Kappeler
Institute of Pathology, University of Bern, Switzerland

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