We use 5% glacial acetic acid in formalin for the trephine biopsy pots so it
is gently decalcifying on receipt then, when fixed (usually left overnight
if received late in day), we transfer it to EDTA solution (50g disod. salt
in 350ml water plus 5g sodium hydroxide) and leave about 8 hours, then
process as normal. Is the recommended protocol in our pathologists' text
book on lymphomas, and routine immunos work well but haven't tried the whole
range so can't guarantee them all

Jacqui Malam
Lancaster



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