I have the Shandon senior, it is a very difficult unit to clean and keep clean as well as having mechanical problems to the extent that Shanon agreed to extend the warranty for an additional year. My opinion is that is over engineered, too much electro mechanical "stuff"mike riceft lauderdale-----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Ofhistonet-request@lists.utsouthwestern.eduSent: Wednesday, July 06, 2005 1:31 PMTo: histonet@lists.utsouthwestern.eduSubject: Histonet Digest, Vol 20, Issue 7Send Histonet mailing list submissions to	histonet@lists.utsouthwestern.eduTo subscribe or unsubscribe via the World Wide Web, visit	http://lists.utsouthwestern.edu/mailman/listinfo/histonetor, via email, send a message with subject or body 'help' to	histonet-request@lists.utsouthwestern.eduYou can reach the person managing the list at	histonet-owner@lists.utsouthwestern.eduWhen replying, please edit your Subject line so it is more specificthan "Re: Contents of Histonet digest..."Today's Topics:   1. RE:no nuclei in frozen sections Last post wrong subject      (Stephen Peters M.D.)   2. Job Opening (Janice A Mahoney)   3. (no subject) (Nava, Josefa)   4. Urgent feedback on MOPEC MB600 VS. Shandon Grosslab	Senior       (Nava, Josefa)   5. microm (Richard Allan) v. Leica glass coverslipper      (Goodwin, Diana)   6. HistoWiki - The Free Histology Encyclopedia - Launched!      (IHC World)   7. goat anti-galectin 1 (Patsy Ruegg)   8. RE: goat anti-galectin 1 (Elizabeth Chlipala)----------------------------------------------------------------------Message: 1Date: Wed, 6 Jul 2005 07:20:31 -0700 (PDT)From: "Stephen Peters M.D." Subject: [Histonet] RE:no nuclei in frozen sections Last post wrong	subjectTo: Histonet@lists.utsouthwestern.eduMessage-ID: <20050706142031.88609.qmail@web30412.mail.mud.yahoo.com>Content-Type: text/plain; charset=iso-8859-1Are the nuclei totally missing? or are there holes in the nuclei? If there are holes in the nuclei I believe this is one of the manifestations of freeze artefact which becomes very obvious in sections in the under 3 micra range. Even though a cryostat is set for 5 micra it is very common for the first few sections to be different thickness, commonly a thick one followed by a thin one. I show pics of what I believe are nuclear ice crystals and the effect of section thickness on this page of my frozen section tutorial:  http://pathologyinnovations.com/new_page_3.htm. If the nuclei are falling out, I have no answer. Stephen ------------------------------Message: 2Date: Wed, 06 Jul 2005 09:40:40 -0500From: "Janice A Mahoney" Subject: [Histonet] Job OpeningTo: Message-ID: Content-Type: text/plain; charset=US-ASCIIHistology TechniciansFull Time/Casual/On-call/Flexible shifts availableAlegent Health is currently looking for a Histology Tech.Registered by American Society of Clinical Pathologists or eligible.  Two years experiencein a clinical laboratory preferred.Alegent (logo)Apply on-line:www.alegent.comPre-employment physical, drug screen and background check required prior to hire.AA/EEOJan MahoneyOmaha NE------------------------------Message: 3Date: Wed, 6 Jul 2005 10:17:58 -0500From: "Nava, Josefa" Subject: [Histonet] (no subject)To: Message-ID:	<2C515C1049EAF5459EFD8C9B929078A41944BF@phdex03.txhealth.org>Content-Type: text/plain;	charset="us-ascii"We are looking to buy a new grossing station and are deciding betweenthe Shandon Grosslab Senior   vs. the MOPEC MB600. Has anyone used theMOPEC MB600 and what do you like/ dislike about  it? I thank you for anyfeedback you can give  us.  Josie NavaPresbyterian Hospital of DallasThe information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law.  If you are not the intended recipient, you are prohibited from copying, distributing, or using the information.  Please contact the sender immediately by return e-mail and delete the original message from your system.------------------------------Message: 4Date: Wed, 6 Jul 2005 10:27:10 -0500From: "Nava, Josefa" Subject: [Histonet] Urgent feedback on MOPEC MB600 VS. Shandon	Grosslab	Senior To: Message-ID:	<2C515C1049EAF5459EFD8C9B929078A41944C0@phdex03.txhealth.org>Content-Type: text/plain;	charset="us-ascii"We  are looking to buy a new  grossing station and are deciding betweenthe Shandon Grosslab Senior vs. the MOPEC MB600. Has anyone used theMOPEC MB600 and  what do you like about it? I thank you for anyinformation you can give us.   Josie NavaPresbyterian Hospital of DallasThe information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law.  If you are not the intended recipient, you are prohibited from copying, distributing, or using the information.  Please contact the sender immediately by return e-mail and delete the original message from your system.------------------------------Message: 5Date: Wed, 6 Jul 2005 11:38:44 -0400 From: "Goodwin, Diana" Subject: [Histonet] microm (Richard Allan) v. Leica glass coverslipperTo: "Histonet (E-mail)" Message-ID:	<992899E9EC268548AB8DDE246AF88473055F51E0@PAHEX01.uphs.upenn.edu>Content-Type: text/plain;	charset="iso-8859-1"Soliciting comments, pro and con, for these two instruments. Thanks! Diana GoodwinSupervisor, Anatomic PathologyPennsylvania Hospital, Philadelphiaph:  215-829-6532fax:  215-829-7564e-mail:  goodwind@pahosp.com ------------------------------Message: 6Date: Wed, 6 Jul 2005 11:50:25 -0400From: "IHC World" Subject: [Histonet] HistoWiki - The Free Histology Encyclopedia -	Launched!To: Message-ID: Content-Type: text/plain;	charset="iso-8859-1"Hello All,Everyone on the Histoland is invited to participate the histology project -HistoWiki - the free-content histology encyclopedia that anyone can edit.The goal of setting up this site is to build the world largest, the mostcomprehensive and up-to-date encyclopedia about everything related toHistology.http://www.ihcworld.com/histowiki/If you have any questions or suggestions, please drop me a email atinfo@ihcworld.comThanks.RichardIHC WorldEmail: info@ihcworld.com------------------------------Message: 7Date: Wed, 6 Jul 2005 09:54:38 -0600From: "Patsy Ruegg" Subject: [Histonet] goat anti-galectin 1To: "'Histonet'" Message-ID: <200507061554.j66FsckM006052@chip.viawest.net>Content-Type: text/plain;	charset="us-ascii"Anybody using SC goat anti-galectin 1 antibody on ffpe tissue? If so pleaseshare your protocol.Thank you,Patsy P.S.Has anyone developed a labelled polymer type detection system to use withgoat primary antibodies yet?------------------------------Message: 8Date: Wed, 6 Jul 2005 10:07:17 -0600From: "Elizabeth Chlipala" Subject: RE: [Histonet] goat anti-galectin 1To: "'Patsy Ruegg'" ,	"'Histonet'"	Message-ID: <000001c58244$c7f20080$a7d48a80@AMY>Content-Type: text/plain;	charset="us-ascii"PatsyI use labeled polymers with goat primaries as follows:After the goat antibody I use a rabbit anti-goat secondary.  I get thisfrom vector labs and then I use the rabbit envision + next.  It worksgreat.  LizElizabeth A. Chlipala, BS, HTL(ASCP)QIHCManagerPremier Laboratory, LLCP.O. Box 18592Boulder, Colorado 80308Office: (303) 735-5001Fax: (303) 735-3540liz@premierlab.comwww.premierlab.com Ship to Address:Premier LaboratoryUniversity of ColoradoMCDB, Room A3B40Boulder, Colorado 80309-----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of PatsyRueggSent: Wednesday, July 06, 2005 8:55 AMTo: 'Histonet'Subject: [Histonet] goat anti-galectin 1Anybody using SC goat anti-galectin 1 antibody on ffpe tissue? If sopleaseshare your protocol.Thank you,Patsy P.S.Has anyone developed a labelled polymer type detection system to usewithgoat primary antibodies yet?_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet------------------------------_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonetEnd of Histonet Digest, Vol 20, Issue 7***************************************----------------------
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