[Histonet] RE: CK 17
We have the Benchmark XT model and, although I haven't tried CK 17 as yet, I
know that there are one or two antisera whose clones it doesn't like despite
trying various protocols. Have you tried any other clones - it might be
worth a go.
Sent: 07 July 2005 18:02
Subject: Histonet Digest, Vol 20, Issue 9
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1. CK17 (Elaine Dooley)
Date: Thu, 07 Jul 2005 12:57:49 -0400
From: "Elaine Dooley"
Subject: [Histonet] CK17
Content-Type: text/plain; charset=US-ASCII
I have been trying to get CK17(M7046) from DAKO to work on the Ventana
Benchmark. I have tried several different tissues for controls. I have
several dilutions and tried several types of retrieval. I get staining but
I also get background that does not seem to dilute out. I have tried DAKO's
background reducing diluent and it helps somewhat.
If anyone uses this antibody on the Ventana Benchmark I wonder if you would
share your protocol? What type of control tissue do you recommend? What
dilution do you use?
For those who use other stainers is there a particular pretreatment that you
Thanks in advance
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