[Histonet] RE: CK 17

From:Malam Jacqueline

We have the Benchmark XT model and, although I haven't tried CK 17 as yet, I
know that there are one or two antisera whose clones it doesn't like despite
trying various protocols. Have you tried any other clones - it might be
worth a go.
Jacqui Malam
Lancaster
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Sent: 07 July 2005 18:02
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 20, Issue 9

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Today's Topics:

   1. CK17 (Elaine Dooley)


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Message: 1
Date: Thu, 07 Jul 2005 12:57:49 -0400
From: "Elaine Dooley" 
Subject: [Histonet] CK17
To: 
Message-ID: 
Content-Type: text/plain;	charset=US-ASCII

Dear Histonetters,

I have been trying to get CK17(M7046) from DAKO to work on the Ventana
Benchmark.  I have tried several different tissues for controls.  I have
several dilutions and tried several types of retrieval.  I get staining but
I also get background that does not seem to dilute out.  I have tried DAKO's
background reducing diluent and it helps somewhat.

If anyone uses this antibody on the Ventana Benchmark I wonder if you would
share your protocol?  What type of control tissue do you recommend? What
dilution do you use? 

For those who use other stainers is there a particular pretreatment that you
recommend?

Thanks in advance

Elaine Dooley



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