[Histonet] RE: CD13 on FFPE tissue

From:"C.M. van der Loos"


   We  too  have experience with Novacastra reagents that doesn't do what
   was promised in the data sheet (CD69 on FFPE). Our problem was finally
   solved  when  we  turned  to  tyramide  amplification (TSA or CSA II).
   Perhaps you can boost your faint CD13 staining in this way.

   Chris van der Loos, PhD
   Dept. of Pathology
   Academical Medical Center M2-230
   Meibergdreef 9
   NL-1105 AZ Amsterdam
   The Netherlands

   phone:  +31 20 5665631
   fax:    +31 20 6960389

   Date: Thu, 7 Jul 2005 15:59:15 +0200
   From: "Andi Kappeler" 
   Subject: [Histonet] CD13 on FFPE tissue
   To: "Histonet" 
   Message-ID: <008401c582fc$21ce60f0$27955c82@patho.unibe.ch>
   Content-Type: text/plain; format=flowed; charset="Windows-1252";
   Hi all
   Has  anybody  experience with CD13 antibodies on FFPE tissues? We have
   Novocastra's  clone 38C12 after different pretreatments (5 variants of
   2  enzyme)  and  are not really enthusiastic about it (faint staining,
   very few
   positive  cells  in  tonsils  and  bone marrow). Suggestions for other
   that work on FFPE welcome! Thanks
   Andi Kappeler
   Insitute of Pathology, University of Bern, Switzerland
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