[Histonet] RE: CD13 on FFPE tissue
We too have experience with Novacastra reagents that doesn't do what
was promised in the data sheet (CD69 on FFPE). Our problem was finally
solved when we turned to tyramide amplification (TSA or CSA II).
Perhaps you can boost your faint CD13 staining in this way.
Chris van der Loos, PhD
Dept. of Pathology
Academical Medical Center M2-230
NL-1105 AZ Amsterdam
phone: +31 20 5665631
fax: +31 20 6960389
Date: Thu, 7 Jul 2005 15:59:15 +0200
From: "Andi Kappeler"
Subject: [Histonet] CD13 on FFPE tissue
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Has anybody experience with CD13 antibodies on FFPE tissues? We have
Novocastra's clone 38C12 after different pretreatments (5 variants of
2 enzyme) and are not really enthusiastic about it (faint staining,
positive cells in tonsils and bone marrow). Suggestions for other
that work on FFPE welcome! Thanks
Insitute of Pathology, University of Bern, Switzerland
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