I have been trying to get CK17(M7046) from DAKO to work on the Ventana
Benchmark. I have tried several different tissues for controls. I have
several dilutions and tried several types of retrieval. I get staining
but I also get background that does not seem to dilute out. I have
tried DAKO's background reducing diluent and it helps somewhat.
If anyone uses this antibody on the Ventana Benchmark I wonder if you
would share your protocol? What type of control tissue do you
recommend? What dilution do you use?
For those who use other stainers is there a particular pretreatment
that you recommend?
Thanks in advance
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