Re: [Histonet] Immunohistochemistry on MMA embedded bone
More than likely, yes. Particularly if your antigen is compromised by
cross linking with formalin. Neil Hand has a publication in Journal of
Histotechnology on doing this. Hopefully, the calcium left in the bone
will not interfer with immunoglobulin reaching an antigenic site/epitope.
If you are looking for murine CD marker, you may be in trouble. In
general, this takes frozen sections for many of the lymphocyte aka CD
markers. You did not say what you are going to stain for??
He used pressure cooker and removed ALL methylmethacrylate, you cannot do
IHC with MMA plastic still in section.
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