Re: [Histonet] Immunohistochemistry on MMA embedded bone

From:Gayle Callis

More than likely, yes.  Particularly if your antigen is compromised by 
cross linking with formalin.   Neil Hand has a publication in Journal of 
Histotechnology on doing this.  Hopefully, the calcium left in the bone 
will not interfer with immunoglobulin reaching an antigenic site/epitope.

If you are looking for murine CD marker, you may be in trouble.  In 
general, this takes frozen sections for many of the lymphocyte aka CD 
markers.  You did not say what you are going to stain for??

He used pressure cooker and removed ALL methylmethacrylate, you cannot do 
IHC with MMA plastic still in section.  

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