Re: [Histonet] ] Gold Chloride for Retic

From:"Alton D. Floyd"

Hello David,

The procedure you are remembering is the generation of gold colloids for
the purpose of performing immunostaining on plastic sections for electron
microscopy.  And you are correct, the color of the scattered light is
indicative of the particle size.  Not absolute, since individual eyes
vary in color perception so much.  Actual size generated depends on the
conditions employed in manufacturing the good particles, which are  then
cleaned by ultracentrifugation prior to use.  The colloid itself is
generated by the protein coating (which can contain the primary
antibodies) which is applied to the particles.

Al Floyd
23126 South Shore Drive
Edwardsburg, MI 49112
(269) 699-7182 phone & fax

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