Re: [Histonet] GFAP Protocol

From:"Jan Shivers"

Sure.  I use Dako's GFAP on their autostainer all the time in veterinary
diagnostics.  It requires NO tissue pretreatment.  The antibody can be used
at a very high dilution (right now I'm using 1:1200 dilution of anti-GFAP
with a DAKO EnVision+/Rb detection system).

Jan Shivers
University of Minnesota Veterinary Diagnostic Lab
St. Paul, MN, USA

----- Original Message ----- 
From: "Onnasch, Hanne" 
Sent: Tuesday, July 20, 2004 3:59 AM
Subject: [Histonet] GFAP Protocol

Hi All,

I'm looking for a protocol for IHC staining for GFAP on paraffin sections.
Does anyone have experience with using the Dako detection kit and an
automated system?


Hanne Onnasch
Research Officer
Central Veterinary Research Laboratory
Dublin 15

phone: +353(0)16072583
Fax: +353(0)16072604

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