Sure.  I use Dako's GFAP on their autostainer all the time in veterinary
diagnostics.  It requires NO tissue pretreatment.  The antibody can be used
at a very high dilution (right now I'm using 1:1200 dilution of anti-GFAP
with a DAKO EnVision+/Rb detection system).

Jan Shivers
University of Minnesota Veterinary Diagnostic Lab
St. Paul, MN, USA
612-624-7297
shive003@tc.umn.edu

----- Original Message ----- 
From: "Onnasch, Hanne" 
To: 
Sent: Tuesday, July 20, 2004 3:59 AM
Subject: [Histonet] GFAP Protocol



Hi All,

I'm looking for a protocol for IHC staining for GFAP on paraffin sections.
Does anyone have experience with using the Dako detection kit and an
automated system?

Regards,
Hanne


Hanne Onnasch
Research Officer
Central Veterinary Research Laboratory
Abbotstown
Castleknock
Dublin 15

phone: +353(0)16072583
Fax: +353(0)16072604
hanne.onnasch@agriculture.gov.ie




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