Re: [Histonet] Endothelial cell staining


The Pharmigen antibody that Joanne mentioned is the best for mouse 
tissue.  Frozen works much better than paraffin sections.  We use thick 
sections (20-30um) to see whole microvascular networks.  Long 
incubations make the results much, much better.  We incubate overnight 
at room temperature!
I don't know if your fixative may have obscured the epitope.  You may 
need to do some antigen unmasking .
Good Luck
Dr.Rocio Sierra-Honigmann
Engineered Wound Repair Laboratory
Cedars Sinai Research Institute
Davis 1091
On Jul 23, 2004, at 8:18 AM, Jeremiah Lyons wrote:

> To whom it may concern,
> I'm a PhD student who is looking for advice on endothelial cell 
> staining in mouse tumour tissue for the purpose of microvessel density 
> staining.Most of the available antibodies don't work in mouse tissue. 
> At the moment I'm trying Carnoy's fixed tissue (fixed for four hours) 
> with rabbit anti-human Von Willebrand factor. Any suggestions would be 
> greatly appreciated.
> Jerry
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