Re: [Histonet] Endothelial cell staining
The Pharmigen antibody that Joanne mentioned is the best for mouse
tissue. Frozen works much better than paraffin sections. We use thick
sections (20-30um) to see whole microvascular networks. Long
incubations make the results much, much better. We incubate overnight
at room temperature!
I don't know if your fixative may have obscured the epitope. You may
need to do some antigen unmasking .
Engineered Wound Repair Laboratory
Cedars Sinai Research Institute
On Jul 23, 2004, at 8:18 AM, Jeremiah Lyons wrote:
> To whom it may concern,
> I'm a PhD student who is looking for advice on endothelial cell
> staining in mouse tumour tissue for the purpose of microvessel density
> staining.Most of the available antibodies don't work in mouse tissue.
> At the moment I'm trying Carnoy's fixed tissue (fixed for four hours)
> with rabbit anti-human Von Willebrand factor. Any suggestions would be
> greatly appreciated.
> Histonet mailing list
Histonet mailing list
<< Previous Message | Next Message >>