Re: [Histonet] APES/positively charged slides

From:Amy Porter

Christie - we dry all of our plastic sections overnight in a 60 C. oven - we have found that this helps retain the sections on the slide through most pretreatments that we use.  Hope this idea helps you out.  

Christie M Quinlan  wrote:Hi all,
I am having problems with plastic sections (MMA) coming off of APES-subbed
slides during deplasticization. I don't want to use gelatin for these as
they are for immuno. Has anyone else encountered this problem? If so,
what method worked for you? Is there any interference with APES solution
and the positive charge on PLUS slides? Thanks in advance for any

Christie Quinlan
W.L. Gore and Assoc.
Flagstaff, AZ

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Amy S.Porter, HT(ASCP) 
Michigan State University 
Department of Physiology 
Division of Human Pathology 
College of Human Medicine

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