RE: [Histonet] water bath adhesives


I'm kinda paranoid about my sections falling of in IHC (antigen 
retrieval can cause them to fall off, and besides IHC takes so 
long that I don't want to have my sections fall off in my last 
water rinse and have wasted all that time) so I both add 1 tsp of 
gelatin to my water bath and coat my slides with    poly-l-lysine. 
The other person who works in my lab has used gelatin in the water 
bath and then plus slides with good results as well. (I don't use 
plus slides mainly because they are more expensive.)
                                Rebekah Smith
On Thu Jul 22 13:53:25 EDT 2004, "Bonner, Janet" 

> We use Sta-on, a Surgipath product, in our waterbathes when using 
> regular
> slides.  We do not use any adhesive in our waterbath (just plain 
> water) when
> using the "Plus" slides - we find this extremely useful for IHC.
> -----Original Message-----
> From: Dorothy.L.Webb@HealthPartners.Com
> [mailto:Dorothy.L.Webb@HealthPartners.Com]
> Sent: Thursday, July 22, 2004 12:00 PM
> To:
> Subject: [Histonet] water bath adhesives
> I am looking into possibly changing the waterbath adhesive and 
> would like to
> get some input from others as to which products you prefer??  
> Also, aren't
> there some of the adhesives on the market that can interfere with 
> certain
> IHC staining?  Thanks ahead of time for all of the help!!  
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