We use to do the same thing, but it got confusing as to which waterbath to use or which slide to use.  Now we only use plus coated slides for everything and no additives in the water.  The cost difference is not that significant to continue using both kinds of slides.  On the other hand, beware of using just one supplier of slides.  They like to get complacent and their QC goes out the drain as well as your sections.  I always rotate ordering from three different vendors. It might sound paranoid, but I've been burned to many times before.  IT DOESN'T MATTER WHO MANUFACTURES THEM, THEY ALL FAIL OCCASIONALLY. No vendors need to reply.  Hope this helps.

Juan C. Gutierrez, HT(ASCP)
Histology Laboratory Supervisor
Christus Santa Rosa Hospital
333 N. Santa Rosa Ave.
San Antonio, TX  78207
(210)704-2533


-----Original Message-----
From: Bonner, Janet [mailto:Janet.Bonner@FLHOSP.ORG]
Sent: Thursday, July 22, 2004 12:53 PM
To: 'Dorothy.L.Webb@HealthPartners.Com';
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] water bath adhesives


We use Sta-on, a Surgipath product, in our waterbathes when using regular
slides.  We do not use any adhesive in our waterbath (just plain water) when
using the "Plus" slides - we find this extremely useful for IHC.

-----Original Message-----
From: Dorothy.L.Webb@HealthPartners.Com
[mailto:Dorothy.L.Webb@HealthPartners.Com]
Sent: Thursday, July 22, 2004 12:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] water bath adhesives



I am looking into possibly changing the waterbath adhesive and would like to
get some input from others as to which products you prefer??  Also, aren't
there some of the adhesives on the market that can interfere with certain
IHC staining?  Thanks ahead of time for all of the help!!  

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