RE: [Histonet] practical exam clarification?

From:"Galbraith, Joe"


I will not speak to the exact current requirements but, when I did my practical for the HTL, I located my own tissue, cut it from the original stored tissue for a signed out case, processed it, embedded it, cut it, stained it, and labeled the slides completely on my own.  Where possible I isolated fresh tissue so I could control fixation as well.  No one did any part of the work other than myself.  Note that you will be graded on tissue selection (meeting the stated criteria), fixation, processing, embedding, cutting, staining, etc so those steps should be your own work, plus you should want to control all these factors yourself and not leave any part of it up to someone else who may not have as high a standard to meet.  However, I must add that I did have the advantage of working in the gross room of a large teaching hospital at the time, so I had access to facilities and tissue sources that many people would not.  This definitely made my task easier.  I do believe that if you get tissue from another institution that you are supposed to acquire fixed tissue and proceed from there.  We have had students from the regional schools who did a practicum at our institution use part of their time with us to find, process and embed tissue here and then take the embedded blocks with them to complete the challenge at their home base. 

Good luck,

Joe Galbraith

-----Original Message-----
[]On Behalf Of
Sent: Tuesday, July 27, 2004 1:10 PM
Subject: [Histonet] practical exam clarification?

Can someone please help with this one?  My supervisor told me that  when 
collecting my tissue I was suppose to cut it by myself... Now, i understand  that 
i have to cut the tissue after processing it and embedding it, but she told  
me i had to cut it from the initial tissue source like our pathologists and our 
 PA'S do.  For example, if i needed a piece of uterus, after the case is =20
signed out, i had to cut sections from that uterus.  Can someone help i'm  sooooo 
confused and i want to make sure i'm doing the right thing.  Thanks  Jennell
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