RE: [Histonet] endothelial cell staining

From:"Elizabeth Chlipala"


You can use PECAM-1 (M-20)  from Santa Cruz.  It is a goat polyclonal
and will work on formalin fixed paraffin embedded material.  I have used
it on mouse tissue with good results.  I have images and a protocol
available if you need one.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Histology Laboratory, LLC
P.O. Box 18592 
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
Ship to Address:
Premier Histology Laboratory
University of Colorado
MCBD, Room A3B40
Boulder, Colorado 80309
This email and any files transmitted with it may contain PRIVILEGED or
CONFIDENTIAL information and may be read or used only by the intended
recipient. If you are not the intended recipient of the email or any of
its attachments, please be advised that you have received this email in
error and that any use, dissemination, distribution, forwarding,
printing, or copying of this email or any attached files is strictly
prohibited. If you have received this email in error, please immediately
purge it and all attachments and notify the sender by reply email or
contact the sender at the number listed above if one is provided. 

-----Original Message-----
[] On Behalf Of Jeremiah
Sent: Friday, July 23, 2004 8:23 AM
Subject: [Histonet] endothelial cell staining


I'm a PhD student who is looking for advice on endothelial cell staining
in mouse tumour tissue for the purpose of microvessel density
staining.Most of the available antibodies don't work on mouse tissue. At
the moment I'm trying Carnoy's fixed tissue (fixed for four hours) with
rabbit anti-human Von Willebrand factor. Any suggestions would be
greatly appreciated.

Histonet mailing list

Histonet mailing list

<< Previous Message | Next Message >>