RE: [Histonet] brightfield 3D reconstruction - COMMERCIAL
We have recently released our TISSUEscope instrument for both fluorescence
and brightfield imaging. Software is available with the system for the
optical sectioning you refer to. [Among other places this fall, the
instrument can be seen at the NSH event in Toronto in September - we'll be
there in conjunction with Beecher Instruments].
However, while we have instruments in our lab setup for confocal detection
in both transmission (brightfield) and fluorescence, the default setup for
detection methods in the TISSUEscope is such that the brightfield images are
acquired in a non-confocal way. I'd be interested in hearing back from you
and others as to the background on this question, usefulness of capability
etc. as we may want to revisit the default setup!
You may also be interested in the first page of an early article on the
subject of confocal transmission imaging that can be seen for free on Nature
bs/351551a0.html - you'll need a subscription to access the pdf for entire
copy. We have hardcopy as well if you'd like a copy - pls contact me
offline if that's the case.
Glenn Smith, P.Eng.
Biomedical Photometrics Inc/GeneFocus
Widefield Confocal Scanning Instruments and Software
Date: Wed, 21 Jul 2004 17:00:33 -0400
From: "Connolly, Brett M"
Subject: [Histonet] brightfield 3D reconstruction
To: "'HISTONET' (email@example.com)"
Aside from 3D reconstruction of confocal, fluorescent images, is anyone
aware of any software/hardware for doing 3D reconstruction on serial
sections of brightfield images? I have heard of VoxBlast from Vaytek, but
that's about it.
Brett M. Connolly, Ph.D.
Merck & Co., Inc.
MRL, Imaging Research
PO Box 4
West Point, PA 19486
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