RE: [Histonet] PGP9.5 staining -- how to get rid of hair follicle background staining?
|From:||"Edmondson David (RBV) NHS Christie Tr" |
Have you tried applying a biotin blocking kit in the procedure?
From: Cao, Xudong [mailto:Xudong_Cao@Brown.edu]
Sent: 23 July 2004 14:02
Subject: [Histonet] PGP9.5 staining -- how to get rid of hair follicle
I am trying to stain for nerve fibers innervating the skin samples that I
have. While I am lucky to get the nerve fiber singnals, I am also getting
overwheelingly strong background staining from the hair follicles. I know
the follicle stains are background since they are also stained in both of my
controls where primary and secondary are omitted are also stained. My
question is: how to get rid of the hair follicle stains? any suggestions? I
am using Vector's ABC and SG substrate. Thanks
Xudong Cao, Ph.D.
Histonet mailing list
<< Previous Message | Next Message >>