RE: [Histonet] Agarose blocks

From:"Charles Scouten"

What kind of microtome are you using?   Soft things need to be cut with a Vibratome.  How thin does it need to be?

Charles W.  Scouten, Ph.D. 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300  
FAX  314 522 0377 

-----Original Message-----
From: [] On Behalf Of Roberta Horner
Sent: Wednesday, July 21, 2004 12:39 PM
Subject: [Histonet] Agarose blocks

I just received tissue in agarose and I tried cutting a couple of them with very little luck.  I have never worked with this before.  Is there a trick to sectioning it?  Should it have been processed differently?  I didn't know what was in the block until I embedded it.

Thank you for any help.

Roberta Horner HT/HTL


Animal Diagnostic Lab

Penn State University

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