[Histonet] theoretical question on fixing and section (formalin and cryosections)

From:"ZP"

Hello all, 

This question refers to bone tissue.  If you want to do IHC, cryosections
generally give better results, right?  Does it make sense to fix the tissue
in 10% formalin, decalcify (say in formic acid), and then "embed" in OCT and
freeze in dry ice to take cryosections?   It just seems like people do 10%
formalin and follow that with paraffin embedding..

Thanks!

Zarana Patel
degaboh@rice.edu


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