I am having problems with the monoclonal p27 antibody. I use super
sensitive strepavadin/biotin kit with citrate antigen retrieval. It is
staining normal neurons.
 
I contracted the manufacture and they suggested to use an non
avidin\biotin kit. I found the non specific staining weaker and if I
increase the dilution, the specific staining was lost.
 
If anyone has any suggestions, I would appreciate.
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