[Histonet] lymph node clearing, hardening

From:"Featherstone, Annette"

Anyone using a homemade lymph node clearing and hardening solution that does
not contain chloroform or ether?
Annette Featherstone

-----Original Message-----
From: Stephen.Eyres@sanofi-synthelabo.com
Sent: Monday, July 19, 2004 09:05
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Bouin's alternative

Hi Marlene,

We use Davidson's fluid, which is a mixture of Alcohol, formalin and acetic



                      Marlene Nardi


                      Sent by:                               cc:

                      histonet-bounces@lists.utsouth         Subject:
[Histonet] Bouin's alternative




                      19/07/2004 13:17



We currently use Bouin's solution to fix animal eyes (good retinal
preservation), to fix animal reproductive tissues (prevents shrinkage of
testes), and as the post-fix in the Masson Trichrome stain.  Any ideas on a
less hazardous alternative solution for any of these uses?  For the
Trichrome stain I have tried the commercial Bouin's substitute, Gram's
iodine and citrate buffer with limited/no success.
thank you in advance,

Marlene C. Nardi
Technical Services Supervisor
Histology Laboratory
New York State College of Veterinary Medicine
Cornell University
Department of Biomedical Sciences
S2-124 Schurman Hall
Ithaca, NY 14853
ph. 607-253-3314 or 607-253-3303
FAX  607-253-3357

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