[Histonet] adhesive tape & MMA Sections - Turpentine?
We have used the Scotch 3M packaging tape #3560 for picking up paraffin
sections from the block. Going to a gelatin subbed slide, and rolled it on
to get rid of water from water bath, clamped the slides together with
plastic between slide, overnight at 37 then overnight at 60C. To remove
this tape, one simply puts it through 3 xylene stations to remove tape
easily and any residual glue. This method was published by Diane Sterchi
in J of Histotechnology. There are different tapes available, simply
trying various brands will tell you is the tape will come off in xylene and
leave section on the slide. This has worked with human femoral bone
sections and also with difficult eyes that want to come apart on a
waterbath. Somewhat fun to do - you have little sections hanging around on
edges until you float them on hotter waterbath, pickup, roll out water,
trim, clamp and dry.
You can pick up PMMA sections the same way by applying tape to block,
sectioning. The hard part will be to get the PMMA embedded bone section to
stick to a gelatin coated slide. I suggest using 275 or 300 bloom in
chrome gelatin mixture and presubbing the slides. Room temperature xylene
should let tape release easily, it may soften the PMMA but that is usually
better removed with warm xylene. Once the tape is off, bone section is on
slide, then you can remove the PMMA with warm xylene.
Instrumedics tape transfer is as Phillip described. I have not heard of a
solvent used to remove tape, unless this is a unique modification of procedure.
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