[Histonet] Can Weil's stain be used on fresh-frozen tissue?


I've found several protocols for "Weil's method" for myelin stain, but they all seem to assume formalin-fixed, paraffin-embedded tissue.  Does anyone know whether it will work in fresh-frozen tissue, perhaps with some formalin fixation of the slide-mounted tissue first?   Thanks for any advice!    Susan Bachus
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