[Histonet] Auramine for Fluoresence
|From:||"Featherstone, Annette" |
Any quik stain for Auramine O Fluorescence for TB?
From: Gayle Callis [mailto:firstname.lastname@example.org]
Sent: Tuesday, July 20, 2004 14:36
To: Featherstone, Annette; Histonet@lists.utsouthwestern.edu
Subject: ] Gold Chloride for Retic
As with all working chemicals, if you exhaust the active ingredient (gold
chloride molecules) it will NOT work properly. Set an expiration date for
your gold chloride, the working solution. OR better yet, set the number of
slides going through this at 50, then make up new. If you start to get
reddish color, then reduce the number of slides going through 50 mls
Concentrated stock solution remains stable for a long time until you make
it up working concentration. We store stock in the refrigerator. If
something lasted "forever", we would have no problems.
We always watched our fibers (where there are the most fibers
located) turn dark blackish gray, I know with Grocotts methenamine silver,
if you leave sections too long in gold chloride they go to a very violet
tinctorial shade, not good - you want blackish-gray. This is the same for
Jones methenamine silver for renal biopsies too. The times are often more
average for toning, some methods give less time but if your eye lets you
control the color development, it helps - run a clock when you do this.
At 08:08 AM 7/19/2004, you wrote:
>I have heard that if the Gold Chloride is old it can cause a more "reddish"
>stain in the retic. Some people are saying it lasts "forever". What is the
This email transmission and any documents, files,
or previous e-mail messages attached to it are
confidential and intended solely for the use of the
individual or entity to whom they are addressed.
If you are not the intended recipient, or a person
responsible for delivering it to the intended recipient,
you are hereby notified that any further review,
disclosure, copying, dissemination, distribution, or
use of any of the information contained in or attached
to this e-mail transmission is strictly prohibited.
If you have received this message in error, please
notify the sender immediately by e-mail, discard
any paper copies, and delete all electronic files
of the message. If you are unable to contact the
sender or you are not sure as to whether you
are the intended recipient, please e-mail
ISTSEC@KaleidaHealth.org or call (716) 859-7777.
Histonet mailing list
<< Previous Message | Next Message >>