[Histonet] Auramine for Fluoresence

From:"Featherstone, Annette"

Any quik stain for Auramine O Fluorescence for TB?
Annette HT/MLT

-----Original Message-----
From: Gayle Callis [mailto:gcallis@montana.edu]
Sent: Tuesday, July 20, 2004 14:36
To: Featherstone, Annette; Histonet@lists.utsouthwestern.edu
Subject: ] Gold Chloride for Retic

As with all working chemicals, if you exhaust the active ingredient (gold 
chloride molecules)  it will NOT work properly.  Set an expiration date for 
your gold chloride, the working solution. OR better yet, set the number of 
slides going through this at 50, then make up new.  If you start to get 
reddish color, then reduce the number of slides going through 50 mls 
working solution.

Concentrated stock solution remains stable for a long time until you make 
it up working concentration.  We store stock in the refrigerator.  If 
something lasted "forever", we would have no problems.

We always watched our fibers (where there are the most fibers 
located)  turn dark blackish gray, I know with Grocotts methenamine silver, 
if you leave sections too long in gold chloride they go to a very violet 
tinctorial shade, not good - you want blackish-gray. This is the same for 
Jones methenamine silver for renal biopsies too.  The times are often more 
average for toning, some methods give less time but if your eye lets you 
control the color development, it helps - run a clock when you do this.

  At 08:08 AM 7/19/2004, you wrote:
>I have heard that if the Gold Chloride is old it can cause a more "reddish"
>stain in the retic. Some people are saying it lasts "forever". What is the
>general consenus?
>Annette Featherstone

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