Re: [Histonet] weird brownish stuff in AEC slides

From:"Katri Tuomala"

Hi Rebekah,
One possibility is formalin pigment, which appears as a brownish black
extracellular pigment, but it would be present also in your negative


Katri Tuomala
St.Joseph's Health Centre
Hamilton, Ontario, Canada
----- Original Message ----- 
Sent: Thursday, July 01, 2004 9:52 AM
Subject: [Histonet] weird brownish stuff in AEC slides

> I have yet another question, guys. I've been asking a lot of
> questions about blocking endogenous peroxidase,but now I have a
> new situation. I'm still using the ABC peroxidase method staining
> sheep placenta (formalin fixed, paraffin embedded) for eNOS and
> using AEC as a chromogen and gill's hematoxylin (blued with tap
> water and water with 2 or 3 drops of ammonia) as a counterstain.
> (btw, still blocking peroxidase with 30% peroxide in methanol,
> since my professors are away and haven't been able to order
> anything better) I have the lovely red and blue contrast in most
> parts of my slides, but I also am getting areas of brownish-black
> staining (kinda DAB colored, although I'm not using any DAB). I'm
> not exactly sure where the brown comes from this and whether its a
> problem with my AEC, my hematoxylin or something else. Anyone have
> any ideas? Thanks in advance, y'all have already been really
> helpful.
> --
> "You are a child of the universe, no less than the trees and the
> stars
> You have a right to be here and whether or not it is clear to you,
> no doubt the universe is unfolding as it should. Therefore be at
> peace with G-d, whatever you conceive Him to be. And whatever your
> labors and aspirations,in the noisy confusion of life, keep peace
> in your soul.-Max Ehrmann,"Desiderata"
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