Re: [Histonet] nissl strain

From:Robert Schoonhoven


Short answer:
What is "happening" is that the tissue on the slide is drying out as the 
xylene evaporates.  The liquid xylene acts similar to a coverslip.

Robert Schoonhoven wrote:

>i did a nissl stain w. cresyl violet.  after my last xylene step i have been
>letting the slides dry off, and i visulize them (w. out a cover slip, these
>slides will be used for LCM), and the slides look great.
>if after about 5 min of drying i look at them, all of a sudden this darkness
>overcomesthe slide and the tissue looks black in color. i can actauly view the
>blackness overcome the slide.
>then if i redip it in xylene, the blackness goes awya and the slides look awsome
>again. i find if i cover slip the slides prior to teh blackness, they preserve
>in the good looking state.  why is this happening? how can i stop it from
>happening w/ our a cover slip?  wat is going on here?
>someone in my lab suggested oxidation, but i am not quite sure wat thta is all
>ann arbor
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