Re: [Histonet] eosin fading

From:"renton louise mrs"

One of the reasons that H&E slides fade is leaving them out in the light. Both fluorescent (some of the lights here are on 24/7) and natural light fade slides to some extent.
  
-----Original Message-----
From: Marshall 
To: histonet@lists.utsouthwestern.edu
Date: Tue, 13 Jul 2004 14:06:17 +0200
Subject: [Histonet] eosin fading

Hi all,
Would like an opinion on what causes eosin to fade while we are on the
fading of hematoxylin debate.  I find that when I stain a batch of
slides 6 months to a year later some of these slides are a lot paler as
regards eosin. I then restain and the slide is fine again. I use a
eosin/phloxine mixture.  Would using an alcoholic eosin mixture improve
this? I am using entellan as mounting medium.
Thanks
sharon marshall
e-mail : marshall@cormack.uct.ac.za
Dept. Human Biology
University of Cape Town
South Africa

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Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
.......so what IS the speed of dark?

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