Re: [Histonet] endogenous alk phos block and HIER
|From:||"SMITH,REBEKAH FELICIA" |
I'm using peroxidase but I do my blocking for endogenous
peroxidase after the AR, and that seems to be working. I have
accidentally reverse it before though, and didn't get wildly
different results, so I'm not sure whether the order matters
On Thu Jul 15 10:46:27 EDT 2004, Amy Porter
> Just curious to know what everyone thinks about pretreatments for
> endogenous products and Heat induced retrieval methods. Do you
> do the blocking before the retrieval or after? I am always
> worried about effecting the pH treatment from the retrieval if i
> pretreat for endogenous after retrieval. On the other hand if I
> block first and then retrieve, am i re-opening what i just tried
> to get rid of while retrieving. I hope this makes sense to
> someone and opinions would be greatly appreciated. I know i will
> get lots of good information, as always - Thanks in advance for
> all the help.
> Amy S.Porter, HT(ASCP) Michigan State University Department of
> Physiology Division of Human Pathology College of Human Medicine
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