I'm using peroxidase but I do my blocking for endogenous 
peroxidase after the AR, and that seems to be working. I have 
accidentally reverse it before though, and didn't get wildly 
different results, so I'm not sure whether the order matters 
much.
                                      Rebekah Smith
On Thu Jul 15 10:46:27 EDT 2004, Amy Porter  
wrote:

> Just curious to know what everyone thinks about pretreatments for 
> endogenous products and Heat induced retrieval methods.  Do you 
> do the blocking before the retrieval or after?  I am always 
> worried about effecting the pH treatment from the retrieval if i 
> pretreat for endogenous after retrieval.  On the other hand if I 
> block first and then retrieve, am i re-opening what i just tried 
> to get rid of while retrieving.  I hope this makes sense to 
> someone and opinions would be greatly appreciated.  I know i will 
> get lots of good information, as always - Thanks in advance for 
> all the help.
> 
> 
> Amy S.Porter, HT(ASCP) Michigan State University Department of 
> Physiology Division of Human Pathology College of Human Medicine 
> portera203@yahoo.com
> 
> 
> 		
> ---------------------------------
> Do you Yahoo!?
> Yahoo! Mail is new and improved - Check it out!
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 



--
SMITH,REBEKAH FELICIA
"You are a child of the universe, no less than the trees and the 
stars
You have a right to be here and whether or not it is clear to you, 
no doubt the universe is unfolding as it should. Therefore be at 
peace with G-d, whatever you conceive Him to be. And whatever your 
labors and aspirations,in the noisy confusion of life, keep peace 
in your soul.-Max Ehrmann,"Desiderata"


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet