Re: [Histonet] OCT und freezing time

From:Gayle Callis

At this temperature, I assume dry ice is cooling the isopentane aka 2 
methyl butane at ~-80C - snap freezing time is basically the same.

At 09:03 AM 7/16/2004, you wrote:
>Hello experts,
>
>I want to freezer my tissue quickly in OCT to preserve
>tissue architecture and macromolecules (RNA, DNA, etc)
>in isopentane at -80C.
>Does anybody know, how much longer you should immerse
>an *OCT-embedded tissue* compared to a *naked tissue* (not OCT-embedded) ?
>
>Thanx in advance.
>
>Cordially,
>
>Ivo.
>
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)



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