Re: [Histonet] Diastase digestion

From:"Katri Tuomala"

Hi Angela,
Are you sure the positive staining in your test slide is glycogen? Liver
cells can have all kinds of endogenous pigments, some of which are Pas


Katri Tuomala
St.Joseph's Health Care
Hamilton, Ontario, Canada

----- Original Message ----- 
From: "Angela Bitting" 
Sent: Thursday, July 08, 2004 11:10 AM
Subject: [Histonet] Diastase digestion

I've got a head-scratcher here.
We stained three human liver cases with  PAS w/ diastase. We mount our
patient tissue on the same slide below the control tissue. We do the
diastase digestion in a Coplin jar and all the slides were in the same jar.
Our Pathologist showed me the slides. The glycogen was removed from the
control tissue, but the test tissue was still positive.
Does anyone have an idea what happened?

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Laboratories

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