Re: [Histonet] Curled Brain Tissue

From:Kathleen Spencer


This is exactly the same problem that we have had for a year now. We 
have tried everything except buying a new cryostat. We also need to do 
c-fos on floating sections. Another guy e-mailed me yesterday with the 
same problem.
He said his sections looked like little burritos sitting in the bottom 
of the well. Ours do too. What kind of cryostat do you have? Also, give 
me as many details as you can, section thickness etc.

Kathleen Spencer HT (ASCP)
Lab Manager/ LCM Supervisor
On Thursday, July 15, 2004, at 12:09  PM, Rob Komorowski wrote:

> Not sure if anyone has any hints for this, but I have a few brains of
> experimental rat brains that were sliced on a cryostat and hat curled, 
> never
> to flatten again. This has made getting an even stain on then very
> difficult. Does anyone have any hints as to how to get them to unroll? 
> I'm
> staining them for c-Fos immuno and they were perfused with 
> paraformaldehyde.
> Thanks for any help!
> Rob K
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