Re: [Histonet] Cholesterol Stains


I don't think it can be done. Cholesterol is a lipid, which dissolves out in
the alcohol and xylene of processing.

If you have any formalin-fixed tissue left, you could do a frozen section,
and then do a lipid stain, or there are a couple of stains that are supposed
to be specific for cholesterol involving oxidizing with ferric salts
(Schultz method comes to mind).

Most of the time, for the FFPE tissues, on the H&E, we look for where the
cholesterol USED TO BE. Sort of like white needles in the pale pink plaque

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073

----- Original Message -----
Sent: Tuesday, July 13, 2004 7:25 PM
Subject: [Histonet] Cholesterol Stains

> Hi,
> I need to do  Cholesterol staining in FFPE tissues.
> Any help would be appreciated.
> Lin
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