Inga,

Did it used to stain spinal cord satifactorily?  If it did and now it
doesn't I would say make it fresh and see if it gets better.

Patsy

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Inga
Hansson
Sent: Monday, May 31, 2004 5:45 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] methyl green


Hi histonetters!

Can anyone explain to me why my methyl green counterstaining stopped
working??

I use 0.5% solution and have had it for a while. Should it be made fresh??
Spinal cord seems to be the least stained! Cerebellum the best. Is
there a difference between different cell types??

Thanks in advance!

Inga

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