RE: [Histonet] changing from high to low profile blades
my mistake...NOT 3 micron......3 degrees
From: Gayle Callis [mailto:firstname.lastname@example.org]
Sent: Thursday, July 15, 2004 9:52 AM
To: Kapoor, Sue; Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] changing from high to low profile blades
Sorry, but 3 micron is NOT the angle, that is the section thickness. The
angle adjustment is on the side of the knife holder usually marked with 0,
5, and 10 with 5 equal to 10 degrees on my Leica.
If you take the bottom slash mark of holder and move it to back, so it
equals around 6 aka 12 degrees or so, keep working with it, you should find
the correct angle for your particular blade. Some blades are better than
others - you can always try another source. Richard Allan Edge Rite are
good, have tried some of Thermo Electrons aka Shandons with success and
Dura Edge - get samples.
With low profiles, depending on manufacturer of blades, you might have
to increase the angle to around 12 degrees or even more - differing from
high profile. I have never found any difference in sharpness between high
and low profiles, but I have found stability differences. We use high
profiles for cryo and regular microtomy in order to cut denser tissue
without chatter and no compression.
At 07:58 AM 7/15/2004, you wrote:
> I have a Reichert-Jung 2030 microtome that I recently changed the back
>plate on so that we can use low profile blades. We're expriencing alot of
>problems in sectioning now, finding it extremely difficult to get a ribbon.
>The sections just compress at the blade. I've tried adjusting the angle,
>had been at 3 micron), didn't seem to help....any suggestions??????!!!???
>Sue Kapoor, HT (ASCP)
>Kenosha Medical Center
>Histonet mailing list
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
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