RE: [Histonet] Xylene recycling

From:"Pamela Marcum"

Hi,

Joyce is right the recycled xylene tends to be purer and work better for
some things and to well for others unless you adjust the processing schedule
for it.   Then you have to watch when you change back to the fresh from the
bottle stuff.  If you only have one processor just be careful as one friend
of mine did years ago just run the freshly opened xylene through the
recycler so it is always the purer form of xylenes.

 Xylenes that we normally use have a number of contaminates in them and we
couldn't afford pure xylene in most cases.

Thanks,

Pam Marcum 


> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Weems,
> Joyce
> Sent: Wednesday, July 07, 2004 9:24 AM
> To: Bell, Lynne; histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] Xylene recycling
>
>
> Recycled xylene is actually purer than new. We have found that
> this can cause GI biopsies to be crisper than when processed with
> new xylene, so we don't use it on our biopsy processor. Also,
> with some coverslippers, new xylene is a must. Other than a bad
> odor that can be remedied with acetic acid, I have had no
> problems with recycled xylene in the 20 yrs (yikes!) I've used it
> - with either of these brands of recyclers.
>
> Good luck!
>
> Joyce
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bell,
> Lynne
> Sent: Wednesday, July 07, 2004 9:07 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Xylene recycling
>
>
> Good Morning All,
>
> Our hospital is demoing a xylene recycler for the next few weeks.  Of
> course, this was done without the lab's knowledge, but that is another
> story for another time.  My question is how pure is the recycled xylene?
> Are you able to use the xylene on the processor as if it were new?  I
> assume that you can use it for staining purposes, but I'm a bit wary
> about using it for processing tissue.  The recycler that is here now is
> from B/R instruments and apparently another one from CBG Biotech will be
> here at the end of the month.
>
>
>
> Any assistance would be greatly appreciated.  Thanks........
>
>
>
> Lynne A. Bell, HT (ASCP)
>
> Central Vermont Hospital
>
> P. O. Box 547
>
> Barre, VT  05641
>
> 802-371-4122
>
>
>
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