RE: [Histonet] Von Kossa staining
I have used this procedure many a time.
If you need a control, immerse one slide in 4.5% citrate buffer for 30-40
Wash all slides in distilled water
Flood slides with 5% silver nitrate
Expose to bright sunlight, or UV light for 20 minutes, or a 60-watt lamp
(about 4-5 inches from the slides) for 30-60 minutes
Wash in several changes of distilled water
Flood slides with 5% sodium thiosulphate for 2-3 minutes
Counterstain with neutral red, safranine or van Gieson.
Clear and mount.
Hope that helps
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