RE: [Histonet] Formalin fix---then freeze?

From:"Houston, Ronnie"

Absolutely; although there is a very real possibility that the tissue will
float off the slide (no matter what type of slide you use) if cutting with a
cryostat. Wash the tissue well before freezing. 
The microtome of choice for sectioning frozen fixed tissue is a freezing
microtome, although there aren't many about any more. What a shame!
that is an art and experience that younger histotechs are losing out on....I
remember the days of free-floating cerebellum
sections................showing my age now.


Ronnie Houston
Director of Anatomic Pathology
Bon Secours HealthPartners Laboratories
5801 Bremo Road
Richmond, VA 23226
(804) 2877972
(804) 2877906 - fax


-----Original Message-----
From: KMIN (Kathy Mink) []
Sent: Monday, July 12, 2004 8:10 PM
Subject: [Histonet] Formalin fix---then freeze?


I have been asked an unusual (stupid?) question and hope someone can help.
Is it possible to formalin fix a tissue and then put it in OCT and freeze it
to cut frozen sections?  I know it doesn't make much sense but apparently a
contract we have was worded this way (obviously not proof read by the
pathologist) and now I need to treat the tissue this way if possible.  Help!

Kathy Mink

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