RE: [Histonet] Fixation and Decalcification of Bone Marrow Bxs.

From:"Patsy Ruegg"

Try using zinc formalin in place of your NBF with the EDTA, it should
improve your H&E morphology.

-----Original Message-----
[]On Behalf Of Nader,
Sent: Sunday, May 16, 2004 11:53 PM
To: Histology Net List Server (E-Mail)
Subject: RE: [Histonet] Fixation and Decalcification of Bone Marrow Bxs.

> About 6 months ago, we switched to fixing our bone marrows
> in 10% NBF saturated with EDTA (this fixes and decals at the
> same time)
> with beautiful results with our IHC and also ISH. Even though our
> pathologists are elated that the IHC and ISH are great, they are not
> completely happy with the morphology of the H&E slides. I was just
> wondering if anyone has any suggestions so we can accomplish
> both great H&E morphology and great stains with IHC and ISH.

What is your formula for the NBF-EDTA mixture? pH?

Alexander Nader MD
Vienna, Austria

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