RE: [Histonet] Antigen retrieval - Microwave problem

From:Margaret Blount

Are You using charged (Superfrost Plus or similar) slides? Also how do you
dry your sections prior to dewaxing. Another point is to bring the buffer to
boiling then turn down the power until it just maintains a gentle simmer, do
not boil the sections vigourously.

That's my two happenyworth.



Margaret Blount
Chief Technician
Clinical Biochemistry
University of Cambridge
Addenbrooke's Hospital
Hills Road
CB2 2QR 

-----Original Message-----
From: Jaimie Hoh []
Sent: Thursday, July 08, 2004 3:36 PM
Subject: [Histonet] Antigen retrieval - Microwave problem

Hi Histonet,

I have a slight problem.

I am perfoming Antigen retrieval using microwave
boiling in Immunocytochemistry.

When I put the slides in the microwave and when the
citrate buffer boils, the specimen comes out from the
slide or it folds.

Does anyone have an alternative to performing Antigen
retrieval using microwave?

Research Officer
Singapore Eye Research Institute

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