jamie, I use to do my retrieval in the microwave and if there was gelatin in 
the baths I had trouble with the tissue coming off.  use + slides with no 
gelatin and you should have no problem.  good luck!   anita dudley, 
providence hosp.  mobile alabama


>From: Jaimie Hoh 
>To: histonet@lists.utsouthwestern.edu
>Subject: [Histonet] Antigen retrieval - Microwave problem
>Date: Thu, 8 Jul 2004 15:36:11 +0100 (BST)
>
>Hi Histonet,
>
>I have a slight problem.
>
>I am perfoming Antigen retrieval using microwave
>boiling in Immunocytochemistry.
>
>When I put the slides in the microwave and when the
>citrate buffer boils, the specimen comes out from the
>slide or it folds.
>
>Does anyone have an alternative to performing Antigen
>retrieval using microwave?
>
>thanks,
>Jaimie
>Research Officer
>Singapore Eye Research Institute
>
>
>
>
>
>___________________________________________________________ALL-NEW Yahoo! 
>Messenger - sooooo many all-new ways to express yourself 
>http://uk.messenger.yahoo.com
>
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_________________________________________________________________
FREE pop-up blocking with the new MSN Toolbar – get it now! 
http://toolbar.msn.click-url.com/go/onm00200415ave/direct/01/


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet