Hi Jaimie,

Try bringing your slides and buffer to just under the boiling point in the microwave and then transferring them to a 93-95 degree C waterbath for the duration.  Better yet, invest in either an electric rice steamer (such as Black & Decker makes here in the states) or an electric laboratory pressure cooker (such as Biocare Medical makes).  The point is to avoid boiling the buffer as it tends to dislodge tissue from the slides.  I assume you are using "plus" or charged slides; this is critical for tissue adherence in immunohistochemistry procedures.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
DM223-VA
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX:  (608)262-7174



-----Original Message-----
From: Jaimie Hoh [mailto:jaimie_hk@yahoo.co.uk]
Sent: Thursday, July 08, 2004 9:36 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Antigen retrieval - Microwave problem


Hi Histonet,

I have a slight problem.

I am perfoming Antigen retrieval using microwave
boiling in Immunocytochemistry.

When I put the slides in the microwave and when the
citrate buffer boils, the specimen comes out from the
slide or it folds.

Does anyone have an alternative to performing Antigen
retrieval using microwave?

thanks,
Jaimie
Research Officer
Singapore Eye Research Institute


	
	
		
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