RE: [Histonet] Antigen retrieval - Microwave problem

From:"Sebree Linda A."

Hi Jaimie,

Try bringing your slides and buffer to just under the boiling point in the microwave and then transferring them to a 93-95 degree C waterbath for the duration.  Better yet, invest in either an electric rice steamer (such as Black & Decker makes here in the states) or an electric laboratory pressure cooker (such as Biocare Medical makes).  The point is to avoid boiling the buffer as it tends to dislodge tissue from the slides.  I assume you are using "plus" or charged slides; this is critical for tissue adherence in immunohistochemistry procedures.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
600 Highland Ave.
Madison, WI 53792
FAX:  (608)262-7174

-----Original Message-----
From: Jaimie Hoh []
Sent: Thursday, July 08, 2004 9:36 AM
Subject: [Histonet] Antigen retrieval - Microwave problem

Hi Histonet,

I have a slight problem.

I am perfoming Antigen retrieval using microwave
boiling in Immunocytochemistry.

When I put the slides in the microwave and when the
citrate buffer boils, the specimen comes out from the
slide or it folds.

Does anyone have an alternative to performing Antigen
retrieval using microwave?

Research Officer
Singapore Eye Research Institute

___________________________________________________________ALL-NEW Yahoo! Messenger - sooooo many all-new ways to express yourself

Histonet mailing list

Histonet mailing list

<< Previous Message | Next Message >>