[Histonet] removing Eosin Staining
After taking off coverslip with xylene, rehydrate the slide - rinsing in
70% or even going back to tap water rinse will take out some eosin. Eosin
is soluble in water and alcohol, if you take out too much eosin, just
restain, preferably with a 70% alcohol rinse BEFORE the eosin, then rinse
with 95% afterwards to differentiate the color.
Overstaining with eosin is a common problem - you may have to adjust your
eosin staining time (less) for her next bunch of slides. The famous
Culling was adamant against too much eosin staining and warned people to
beware - make it light, with 5 to 6 different shades of red to
pink (different tissue components will have a different red or tinctorial
level) - and he felt most pathologists and other people were looking H&E
with eosin much too red.
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