[Histonet] protocol for freezing fixed rat skeletal muscle
Thanks for the response to my question "mapping capillary perfusion pattern".
Here I have another question need your help. I am going to perfuse rat
hindlimb with BSA+FITC-dextran, then to locate the FITC-dextran in frozen
muscle tissue. To prevent FITC-dextran from diffusing out of tissue when I
mount the tissue onto a slide, will some fixation before freezing the
tissue be helpful? Can anyone kindly share a good protocol for freezing
fixed muscle tissue? fixative? how long the fixation? any cryoprotection?
By the way, I wonder if anyone knows whether the FITC will bleach during
the fixation, freezing process? Thanks in advance.
Biochemistry, medicine school
University of Tasmania, Australia
61 03 6226 2669
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