[Histonet] eosin fading


Hi all,
Would like an opinion on what causes eosin to fade while we are on the
fading of hematoxylin debate.  I find that when I stain a batch of
slides 6 months to a year later some of these slides are a lot paler as
regards eosin. I then restain and the slide is fine again. I use a
eosin/phloxine mixture.  Would using an alcoholic eosin mixture improve
this? I am using entellan as mounting medium.
sharon marshall
e-mail : marshall@cormack.uct.ac.za
Dept. Human Biology
University of Cape Town
South Africa

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