[Histonet] endogenous alk phos block and HIER

From:Amy Porter

Just curious to know what everyone thinks about pretreatments for endogenous products and Heat induced retrieval methods.  Do you do the blocking before the retrieval or after?  I am always worried about effecting the pH treatment from the retrieval if i pretreat for endogenous after retrieval.  On the other hand if I block first and then retrieve, am i re-opening what i just tried to get rid of while retrieving.  I hope this makes sense to someone and opinions would be greatly appreciated.  I know i will get lots of good information, as always - Thanks in advance for all the help.

Amy S.Porter, HT(ASCP) 
Michigan State University 
Department of Physiology 
Division of Human Pathology 
College of Human Medicine 

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