[Histonet] curled sections
For those trying to cut thick cryosections, it is not uncommon for them
to curl. If you are going to run IHC on floating sections just place
them into your post -fix or PBS if they are fixed, right when you cut
them. They will flatten out. Run your stains per floating protocol. When
done developing, float in a staining dish of distilled water and mount
onto charged slides, airdry completely, dip into xylene, coverslip, done.
It works well for me. Questions, call me.
U of MN
Division of Neuropathology
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