For those trying to cut thick cryosections, it is not uncommon for them 
to curl. If you are going to run IHC on floating sections just place 
them into your post -fix or PBS if they are fixed, right when you cut 
them. They will flatten out. Run your stains per floating protocol. When 
done developing, float in a staining dish of distilled water and mount 
onto charged slides, airdry completely, dip into xylene, coverslip, done.

It works well for me. Questions, call me.

Colleen Forster
U of MN
Division of Neuropathology
612-626-0436


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