[Histonet] best fixative for EM_IHC

From:"Sandeep Gopalakrishnan"

   hi histonetters,
   Could  any  one give a best= fixative for IHC work in bone sections
   for  electron  microscopy  studies.  I  =  am  fixing presently in the
   karnovsky's  fixative.  Is  that  a best one? Simila= rly Which buffer
   (cacodylate   or  phhospahte  buffer  )  is  the  best  to  prepare     karnovsky's  fixative.  Since  cacodylate  is  stable  i am  presently
   using= it. Is there any other suggestions.
   thanks in advance.
   = Sandeep gopalakrishnan
   Sandeep Gopalakrishnan

   = Research Assistant

   Transmission Electron Microscopy Labora= tory

   Division of Implant Biology

   Biomedical Technolo= gy Wing 

   Satelmond palace campus

   Sree Chitra&nb= sp;Tirunal Institute for Medical Sciences 

   Poojapura, Tr= ivandrum: 695012

   Kerala state,India


   Phone:= +91-471-2340801(extension:215)

   Cell :9846387770



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