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                      western.edu                           To:       histonet@lists.utsouthwestern.edu                                             
                      05/07/04 03:05 AM                     Subject:  Histonet Digest, Vol 8, Issue 8                                               
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Today's Topics:

   1. pneumocystis by polarization (RSRICHMOND@aol.com)
   2. RE: CAP and Computer Training (Valerie Biendara)


Message: 1
Date: Sat, 3 Jul 2004 14:18:18 EDT
From: RSRICHMOND@aol.com
Subject: [Histonet] pneumocystis by polarization
To: histonet@lists.utsouthwestern.edu
Message-ID: <98.edae86b.2e18526a@aol.com>
Content-Type: text/plain; charset="US-ASCII"

Sue O'Brien, Histology Supervisor, at Burdette Tomlin Memorial Hospital, Cape
May Court House, New Jersey, asks:

>>I was wondering if anyone else had tried Dr. Teisa An's method (published
in the March 2004 issue of Arch. Pathol. Lab. Med.)? It utilizes pepsin, and
then the PCP can be visualized via polarization. For those that have tried it,
what do you think? It is very easy to do the procedure, but the results seem a
little different (for someone used to looking at silver stained material).<<

The article is "The use of polarization microscopy in the diagnosis of
pneumocystis pneumonia" by Teisa An MD and Pam Tabaczka BS(MT) in the pathology
department at Harper University Hospital in Detroit MI (Dr. An's e-mail is tan
dmc.org.), in Arch Pathol Lab Med 2004;128:163-4. They write "When tissue
sections that had been treated with pepsin and stained with hematoxylin only
examined under polarized light, the cyst forms of P carinii were observed as
being clearly birefringent and were as numerous as when a Grocott methenamine
silver-stained slide was examined."

This article is a good example of why I get the Archives because the College
of American Pathologists sends it to me, but usually let it accumulate on top
of the previous month's number. The authors report exactly one case, without
even noting whether a pneumocystis control slide was positive, how they applied
the pepsin, or what hematoxylin they used. They offer no relevant references,
and no explanation of how the method might work. They don't mention that the
present name of the human pathogen is Pneumocystis jiroveci. If the Archives
is actually a refereed journal, then taunts about the umpire's seeing-eye dog
are definitely in order.

Still, it couldn't hurt to try the method, but it's hardly ready for prime

Bob Richmond
Samurai Pathologist
Knoxville TN and Gastonia NC


Message: 2
Date: Fri, 2 Jul 2004 20:31:18 -0500
From: "Valerie Biendara" 
Subject: RE: [Histonet] CAP and Computer Training
Message-ID: <20040703013142.KIWX14781.fe6@Valerie>
Content-Type: text/plain;            charset="US-ASCII"

See CAP Checklist, Laboratory General

GEN.44500             Phase II             N/A   YES   NO

Is there documentation that all users of the computer system receive
adequate training initially, after system modification, and after
installation of a new system?


The people who interact with the computer system must initially be taught
how to use a new system and must be trained on modifications to an existing
system.  There must be documentation of these training activities.

I'm preparing for my CAP for the Fall and I don't see anything mentioned
about computer training. If there is something, please let me know. I must
be missing it.

We have been going round and round over this. Someone has said that CAP
requires semi-annual refresher training on the computer system, but we
can't find any documentation. Help!!


Victor Tobias
Clinical Applications Analyst
Dept of Pathology
University of Washington Medical Center
206-598-7659 Fax

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