[Histonet] Re: [IHCRG] CD31 on porcine

From:Philip Seifert


We've succesfully used Serotec's antibody to Porcine CD31 on paraffin and resin
embedded tissues.  Here are some research article references;

Nugent HM, Groothuis A, Seifert P, Guerraro JL, Nedelman M, Mohanakumar T,
Edelman ER. Perivascular endothelial implants inhibit intimal hyperplasia in a
model of arteriovenous fistulae: a safety and efficacy study in the pig.
Journal of Vascular Research. 2002 Nov-Dec;39(6):524-33.

Nugent HM, Edelman ER. Endothelial implants provide long-term control of
vascular repair in a porcine model of arterial injury. Journal of Surgical
Research. 2001 Aug. 99(2): 228-34. 

Use pressure cooker-Antigen retrieval for 20 minutes (High pH is optimal though
section adherance is a problem) or low temp water bath for 2-3 hours @ 80oC.  I
would suggest you use Dakocytomation CSAII or Envision+ for detection
antigenicity is low for porcine CD31 in 10%NBF fixed tissues.


Philip Seifert, MS, HTL(ASCP)
Research Associate
Massachusetts Institute of Technology
Biomedical Engineering Center Histology Unit
Building 16-336
77 Massachusetts Avenue
Cambridge, MA 02139

E|   paseifer@mit.edu
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Quoting Patsy Ruegg :

> Does anyone know of a CD31 or CD34 procedure that will work on ffpe pig
> tissue?
> Patsy
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