[Histonet] RE: curl sections
For those trying to cut thick cryosections, it is not uncommon for them
to curl. Just place them into your post -fix floating sections or PBS if
they are fixed, right when you cut them. They will flatten out. Mount
onto charged or gelatin coated slides. Air-dry for a minutes ant then
run your Stains or immuno previous bath in distilled water. Per floating
protocol lose a lot of regents.. When done dip into alcohols, xylene,
coverslip.
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